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Jingyue Ju

Samuel Ruben-Peter G. Viele Professor of Engineering
Chemical Engineering
Northwest Corner Building - Room 806, 550 West 120th Street
  • Director, Center for Genome Technology & Biomolecular Engineering

Our laboratory focuses on the design and synthesis of novel molecular tags for biological labeling and imaging, and developing new technologies to study problems in genomics. We are pursuing these research objectives by using chemical science, engineering principles and experimental biological approaches. We have pioneered the use of chemistry and fluorescence energy transfer (ET) principles to construct ET molecular tags for high-throughput genomic research. The ET tags facilitated the rapid development of laser induced fluorescence capillary-array DNA sequencers, which are the major driving force for completion of the international Human Genome Project.

We have invented a Combinatorial Fluorescence Energy Transfer (CFET) Labeling Approach that uses a limited number of fluorescent molecules to create a maximum number of fluorescent tags that have unique fluorescence signatures. We are studying the chemical and physical properties of these CFET tags using state-of-the-art instrumentation, and applying the CFET tags to cellular imaging, multiplex DNA sequencing, genetic mutation detection, genomewide chromosome deletion and insertion analysis. We have also invented a novel DNA sequencing chemistry using solid phase capturable dideoxynucleotides and mass spectrometry. This method produces accurate and digital genetic sequence information with very high speed. We are currently developing integrated nanoscale systems to couple with the solid phase DNA sequencing chemistry for detecting disease-related mutations on a genomic scale. These technologies coupled with improvements of the conventional technologies are being implemented in large-scale genomic sequencing projects and disease gene discovery projects at the Columbia Genome Center. Two other inventions from our group involve the use of photochemistry, enzyme catalysis, mass spectrometry, and microfabrication for high throughput parallel imaging of DNA/RNA sequences in chip and nanoscale devices. These research projects are targeted to fulfill the requirement of personalized medicine We are also engaged in projects to design and synthesize novel biopolymer analogues with programmed detection and biological properties for high-throughput research in bioscience and genomics.

Research & Other Works
Diagnostic system for rapid and sensitive differential detection of pathogens
MassTag polymerase chain reaction for differential diagnosis of viral hemorrhagic fevers
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy
Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry
Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences
Molecular beacons with intrinsically fluorescent nucleotides
Spectroscopic investigation of a FRET molecular beacon containing two fluorophores for probing DNA/RNA sequences
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